Illumination scheme for superresolution microscopy is developed. The scheme accomplishes dark filed illumination with a laser light source including spatial coherence suppression. The scheme allows to observe nanoparticles with a size smaller than 50 nm. This is necessary to get higher resolution in the previously proposed method of superresolution microscopy (Near field Optical Random Microscopy – NORM). This method is based on real-time video processing of a nanoparticles Brownian motion those are located near the object surface. The method of vertical coordinate measurement is demonstrated. This method is based on astigmatic nanoparticle imaging. Three-dimensional distributions of suspended nanoparticles are obtained. Vertical resolution better than 200 nm and lateral resolution better than 100 nm are demonstrated.
Author Biographies
Sergey Aleksandrovich Asselborn, South Ural State University, Chelyabinsk
Cand. Sc. (Physics and Mathematics), Senior Staff Scientist, Laboratory of Sensorics
Evgeniy Sergeevich Zatsepin, South Ural State University, Chelyabinsk
Laboratory Assistant-Researcher, Laboratory of Sensorics
Denis Sergeevich Isakov, South Ural State University, Chelyabinsk
Researcher, Laboratory of Sensorics
Aleksandr Mikhaylovich Gerasimov, South Ural State University, Chelyabinsk
Cand. Sc. (Physics and Mathematics), Associate Professor, Department of “Optoinformatics”, Senior Staff Scientist, Laboratory of Sensorics
Denis Grigor'evich Pikhulya, South Ural State University, Chelyabinsk
Cand. Sc. (Physics and Mathematics), Associate Professor, Department of “Optoinformatics”, Senior Staff Scientist, Laboratory of Sensorics
Yuriy Vladimirovich Miklyaev, South Ural State University, Chelyabinsk
Dr. Sc. (Physics and Mathematics), Professor, Department of Physics of Nanoscale Systems, Leading Researcher, Laboratory of Sensorics
