ОБОГАЩЁННАЯ МИКРОБНАЯ БИОМАССА: СТАБИЛЬНОСТЬ И ПОТЕНЦИАЛЬНАЯ БИОДОСТУПНОСТЬ ЦЕЛЕВЫХ МЕТАБОЛИТОВ

Rinat I. Fatkullin; Irina V. Kalinina; Natalia V. Naumenko

Authors

Rinat I. Fatkullin South Ural State University, Chelyabinsk, Russia
Irina V. Kalinina South Ural State University, Chelyabinsk, Russia
Natalia V. Naumenko South Ural State University, Chelyabinsk, Russia

Keywords:

microbial biomass, distillery stillage, ergosterol; vitamin B₁₂, thermal stability, in vitro digestion, potential bioavailability

Abstract

Biologically active compounds produced by microorganisms represent a promising re-source for the development of functional food ingredients. The present study was aimed at evaluating the functional potential of microbial biomass enriched with biologically active compounds. Saccha-romyces cerevisiae and Propionibacterium freudenreichii were used as producing microorganisms. The microorganisms were cultivated on complex nutrient media containing components of distillery stillage. Ergosterol, a precursor of vitamin D₂, and vitamin B₁₂ were considered as the target metabo-lites and biologically active compounds. Compared with the control cultivation conditions, the dry bi-omass yield of S. cerevisiae increased from 8.7 to 10.9 g/L, while the ergosterol content increased from 0.82 to 1.21%. For propionic acid bacteria, the biomass yield increased from 6.8 to 8.0 g/L, while the vitamin B₁₂ content increased from 43.6 to 72.4 μg/g of dry biomass. At the next stage, the potential stability of the biologically active compounds within the microbial biomass was evaluated under elevated-temperature treatment. Ergosterol showed higher thermal stability: after treatment at 100 °C for 5 min, 84.3% of its initial amount was retained, whereas the corresponding value for vita-min B₁₂ was 68.8%. Under simulated gastric and intestinal digestion, ergosterol retention was 93.4 and 86.0%, while vitamin B₁₂ retention was 89.4 and 80.0%, respectively. The results demonstrated the potential of enriched microbial biomass as a source of biologically active compounds capable of retaining a substantial proportion of their initial content during technological processing and simulat-ed gastrointestinal digestion.

Author Biographies

Rinat I. Fatkullin, South Ural State University, Chelyabinsk, Russia

Candidate of Technical Sciences, Associate Professor, Department of Food Science and Biotechnology

Irina V. Kalinina, South Ural State University, Chelyabinsk, Russia

Doctor of Technical Sciences, Professor, Associate Professor, Department of Food Science and Biotechnology

Natalia V. Naumenko, South Ural State University, Chelyabinsk, Russia

Doctor of Technical Sciences, Associate Professor, Department of Food Science and Biotechnology

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