BIOLOGICAL EFFECTS AND MAIN MECHANISMS OF INFLUENCE OF ELECTROLYZED RECONSTITUTED WATER ON HUMANS

Authors

  • Oleg A. Suvorov Federal State Budgetary Institution of Science “Institute of Theoretical and Experimental Biophysics” of the Russian Academy of Sciences (Pushchino, Moscow region)
  • Artyom I. Panait Federal State Budgetary Institution of Science “Institute of Theoretical and Experimental Biophysics” of the Russian Academy of Sciences (Pushchino, Moscow region)
  • Svetlana Yu. Voloshaninova Federal State Budgetary Institution of Science “Institute of Theoretical and Experimental Biophysics” of the Russian Academy of Sciences (Pushchino, Moscow region)
  • Alexander L. Kuznetsov Federal State Budgetary Institution of Science “Institute of Theoretical and Experimental Biophysics” of the Russian Academy of Sciences (Pushchino, Moscow region)
  • Larisa G. Ipatova Federal State Budgetary Institution of Science “Institute of Theoretical and Experimental Biophysics” of the Russian Academy of Sciences (Pushchino, Moscow region)
  • Alexander G. Pogorelov Federal State Budgetary Institution of Science “Institute of Theoretical and Experimental Biophysics” of the Russian Academy of Sciences (Pushchino, Moscow region)

Keywords:

electrochemically activated water, electrolyzed reduced water, oxidative stress, antioxidant, mechanism

Abstract

The formation of reactive oxygen species or free radicals causes oxidative damage to biological macromolecules, which contributes to the development of various diseases and rapid aging of the body. The effect of reducing oxidative stress was demonstrated when using reduced electrolyzed water obtained during electrochemical activation of water in the area adjacent to the cathode. Reduced water is saturated with molecular hydrogen, which has antioxidant properties, it is characterized by alkaline pH values and negative values of redox potential. The bioactivity of the reduced electrolysis water and its antioxidant efficiency are shown. 70 % of the microbial species listed in the human Microbiome project are able to metabolize hydrogen, which confirms its role in the physiology of the intestinal microflora. However, further study of the effect of electrolyzed reduced fraction (ERF) on the intestinal microbiome is required to understand the mechanisms of beneficial effects of electrolyzed reduced water on human health. The increased antioxidant activity of the electrolyzed reduced water can be explained by the presence of active hydrogen in the water. However, other properties and effectiveness of the recovered water have yet to be determined. Further intensive characterization of ERF is necessary to determine its actual recovery capacity. In addition, the effects of ERF on human physiology should be further investigated in detail, given that about 70 % of the human body is water. The results of published studies show that daily use of ERF can improve health and physical performance even in healthy people who do not have gastrointestinal symptoms.

Author Biographies

Oleg A. Suvorov, Federal State Budgetary Institution of Science “Institute of Theoretical and Experimental Biophysics” of the Russian Academy of Sciences (Pushchino, Moscow region)

Ph.D, Employee, Laboratory of functional microscopy of biostructures

Artyom I. Panait, Federal State Budgetary Institution of Science “Institute of Theoretical and Experimental Biophysics” of the Russian Academy of Sciences (Pushchino, Moscow region)

Junior Researcher, Laboratory of functional microscopy of biostructures

Svetlana Yu. Voloshaninova, Federal State Budgetary Institution of Science “Institute of Theoretical and Experimental Biophysics” of the Russian Academy of Sciences (Pushchino, Moscow region)

Employee, Laboratory of functional microscopy of biostructures

Alexander L. Kuznetsov, Federal State Budgetary Institution of Science “Institute of Theoretical and Experimental Biophysics” of the Russian Academy of Sciences (Pushchino, Moscow region)

Ph.D, Employee, Laboratory of functional microscopy of biostructures

Larisa G. Ipatova, Federal State Budgetary Institution of Science “Institute of Theoretical and Experimental Biophysics” of the Russian Academy of Sciences (Pushchino, Moscow region)

Ph.D, Employee, Laboratory of functional microscopy of biostructures

Alexander G. Pogorelov, Federal State Budgetary Institution of Science “Institute of Theoretical and Experimental Biophysics” of the Russian Academy of Sciences (Pushchino, Moscow region)

Ph.D, Head, Laboratory of functional microscopy of biostructures

References

Henry M., Chambron J. Physico-chemical, biological and therapeutic characteristics of electrolyzed reduced alkaline water (ERAW). Water, 2013, vol. 5, pp. 2094–2115. DOI: 10.3390/w5042094.

Бахир В.М. Электрохимическая активация. М.: Вива-Стар, 2014. 511 с. [Bahir V.M. Elektrokhimicheskaya aktivatsiya [Electrochemical activation]. Moscow, 2014. 511 p.].

Takenouchi T., Wakabayashi S. I. Rinsing effect of alkaline electrolyzed water on nickel surfaces. Journal of Applied Electrochemistry, 2006, vol. 36, no. 10, pp. 1127–1132. DOI: 10.1007/s10800-006-9196-x.

Kim C., Hung Y.C., Brackett R.E. Robert E. Roles of Oxidation–Reduction Potential in Electrolyzed Oxidizing and Chemically Modified Water for the Inactivation of Food-Related Pathogens. Journal of Food Protection, 2000, vol. 63, no. 1, pp. 19–24. DOI: 10.4315/0362-028x-63.1.19.

Nishikawa R., Teruya K., Katakura Y., Osada K., Hamasaki T., Kashiwagi T. Effect of acidic electrolyzed water on the viability of bacterial and fungal plant pathogens and on bacterial spot disease of tomato. Electrolyzed Reduced Water Supplemented with Platinum Nanoparticles Suppresses Promotion of Twostage Cell Transformation. Cytotechnology, 2005, vol. 47, no. 1-3, pp. 97–105. DOI: 10.1007/s10616-005-3759-2.

Stopforth J.D., Mai T., Kottapalli B., Samadpour M. Effect of Acidified Sodium Chlorite, Chlorine, and Acidic Electrolyzed Water on Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes Inoculated onto Leafy Greens. Journal of Food Protection, 2008, vol. 71, no. 3, pp. 625–628. DOI:

4315/0362-028x-71.3.625.

Park E.J., Alexander E., Taylor G.A., Costa R., Kang D.H. Effect of Electrolyzed Water for Reduction of Foodborne Pathogens on Lettuce and Spinach. Journal of Food Science, 2008, vol. 73, no. 6, pp. 268–272. DOI: 10.1111/j.1750-3841.2008.00809.x.

Koseki M., Tanaka Y., Noguchi H., Nishikawa T. Effect of pH on the Taste of Alkaline Electrolyzed Water. Journal of Food Science, 2007, vol. 72, no. 5, pp. 298–302. DOI: 10.1111/j.1750-3841.2007.00384.x.

Bulychev N.A., Kazaryan M.A., Kirichenko M.N., Garibyan B.A., Morozova E.A., Chernov A.A. Obtaining of hydrogen in acoustoplasma discharge in liquids. Proceedings of SPIE, 2018, Vol. 10614, article number 1061411-1. DOI: 10.1117/12.2303452

Gómez-Espinosa D., Cervantes-Aguilar F. J., Río-García D., Carlos J., Ameliorative effects of neutral electrolyzed water on growth performance, biochemical constituents, and histopathological changes in Turkey poults during aflatoxicosis. Toxins, 2017., vol. 9, no. 3, pp. 104–113. DOI: 10.3390/toxins9030104.

Audenaert K., Monbaliu S., Deschuyffeleer N., MaeneP., VekemanF., Haesaert G., De Saeger S., Eeckhout M. Neutralized electrolyzed water efficiently reduces Fusarium spp. in vitro and on wheat kernels but can trigger deoxynivalenol (DON) biosynthesis. Food Control., 2012, vol. 23, pp. 515–521.DOI:

1016/j.foodcont.2011.08.024

Han A.L. Hydrogen treatment protects against cell death and senescence induced by oxidative damage. J Microbiol Biotechnol., 2017, vol. 27, pp. 365–371.

Ohsawa I., Ishikawa M., Takahashi K., Watanabe M., Nishimaki K., Yamagata K., Katsura K., Katayama Y., Asoh S., Ohta S. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic

oxygen radicals. Nat Med., 2007, vol. 13, no. 10, pp. 1038–1577. DOI: 10.1038/nm1577

Lee M.Y., Kim Y.K., Ryoo K.K., Lee Y.B., Park E.J. Electrolyzed-reduced water protects agains oxidative damage to DNA, RNA, and protein. Appl Biochem Biotechno, 2006, vol. 135, pp. 133–144. DOI:

1385/abab:135:2:133.

Ridwan R.D. The Ability of Electrolyzed Reduced Water to Act as an Antioxidant and AntiInflammatory Agent in Chronic Periodontitis Wistar Rats (Rattus novergicus). Kafkas Universitesi Veteriner

Fakultesi Dergisi, 2019, vol. 4, pp. 1–6. DOI: 10.9775/kvfd.2018.21284.

Nakayama M., Kabayama S., Ito S. The hydrogen molecule as antioxidant therapy: Clinical application in hemodialysis and perspectives. Renal Replacement Therapy, 2016, vol. 2, pp. 23–29. DOI: 10.1186/s41100-016-0036-0.

Choi W., Hee-Souk K., Hyeon Y.L. Enhancement of anti-skin inflammatory activities of scutellaria baicalensis using an alkaline reduced water extraction process. MSc Thesis, Department of Medical Biomaterials Engineering, Kangwon National University, Chuncheon, Gangwon, Korea. 2014, vol. 12, pp. 700–711.

Mahmud M.F., Mohammed S.F. Effect of ionized water and percentage of concentrate on the rumen environment and some of blood characteristics of iraqi lambs. Biochem. Cell. Arch., 2019, vol. 19, no. 1, pp. 1487–1492.

Arifah RA: The effect of alkaline drinking water to increase the number of plasma cells in chronic inflammation. Undergraduate Thesis, Fakultas Kedokteran Gigi. Universitas Airlangga Surabaya. 2015, vol. 14, pp. 35–47.

Harper A., Naghibi M., Garcha D. The Role of Bacteria, Probiotics and Diet in Irritable Bowel Syndrome. Foods, 2018, vol. 7, no. 2, p. 13. DOI: 10.3390/foods7020013.

Shin D.W., Yoon H., Kim H.S., Choi Y.J., Shin C.M., Park Y. Effects of alkaline-reduced drinking water on irritable bowel syndrome with diarrhea: a randomized double-blind, placebo-controlled pilot study.

Evidence-Based Complementary and Alternative Medicine, 2018, vol. 13, pp. 8–16. DOI: 10.1155/2018/9147914.

Harris L.A., Baffy N. Modulation of the gut microbiota: a focus on treatments for irritable bowel syndrome. Postgraduate Medical Journal, 2017, vol. 129, no. 8, pp. 872–888. DOI:

1080/00325481.2017.1383819.

Yoshida N., Takemura T., Granger D.N., Anderson D.C., Wolf R.E., McIntire L.V., Kvietys P.R. Molecular determinants of aspirin-induced neutrophil adherence to endothelial cells. Gastroenterology,

, vol. 105, pp. 715–724. DOI: 10.1016/0016-5085(93)90888-J

Remig V., Franklin B., Margolis S., Kostas G., Nece T. and Street J.C. Trans fats in America: a review of their use, consumption, health implications, and regulation. J. Am. Diet. Assoc., 2010, vol. 110, pp. 585–592. DOI: 10.1016/j.jada.2009.12.024.

Viner R.M., Hsia Y., Tomsic T. and Wong I.C. Efficacy and safety of anti-obesity drugs in children and adolescents: systematic review and metaanalysis. Obes. Rev., 2010, vol. 11, pp. 593–602.

Tanaka Y., Saihara Y., Izumotani K., & Nakamura, H. Daily ingestion of alkaline electrolyzed water containing hydrogen influences human health, including gastrointestinal symptoms. Medical gas research, 2018, vol. 8, pp. 160–167. DOI: 10.4103/2045-9912.248267.

Ohno K., Ito M., Ichihara M., Ito M. Molecular hydrogen as an emerging therapeutic medical gas for neurodegenerative and other diseases. Oxid Med Cell Longev, 2012, vol. 35, pp. 31–42. DOI:

1155/2012/353152.

Higashimura Y., Baba Y., Inoue R., Takagi Т., Uchiyama К., Mizushima K. Effects of molecular hydrogen-dissolved alkaline electrolyzed water on intestinal environment in mice. Medical gas research, 2018, vol. 8, pp. 6–12. DOI: 10.4103/2045-9912.229597.

Kim C., Hung Y. C., Brackett R.E. Efficacy of electrolyzed oxidizing (EO) and chemically modified water on different types of foodborne pathogens. Source: International Journal of Food Microbiology, 2000, vol. 61, no. 2, pp. 199–207. DOI: 10.1016/S0168-1605(00)00405-0.

Deza M.A., Araujo M., Garrido M.J. Efficacy of Neutral Electrolyzed Water To Inactivate Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, and Staphylococcus aureus on Plastic and Wooden Kitchen Cutting Boards. Source: Journal of Food Protection, 2007, vol. 70, no. 1, pp. 102–108.

DOI: 10.4315/0362-028X-70.1.102.

Jardon-Xicotencatl S., Díaz-Torres R., Marroquín-Cardona A., Villarreal-Barajas T., MéndezAlbores A. Detoxification of aflatoxin-contaminated maize by neutral electrolyzed oxidizing water. Toxins,

, vol.7, pp. 4294–4314. DOI: 10.3390/toxins7104294

Escobedo-González R., Méndez-Albores A., Villarreal-Barajas T., Aceves-HernándezJ.M., Miranda-Ruvalcaba R., Nicolás-Vázquez I. A theoretical study of 8-chloro-9-hydroxy-aflatoxin B1, the

conversion product of aflatoxin B1 by neutral electrolyzed water. Toxins, 2016, vol. 8, pp. 225–238. DOI: 10.3390/toxins8070225.

Ito H., Kabayma S., Goto K. Effects of electrolyzed hydrogen water ingestion during endurance exercise in a heated environment on body fluid balance and exercise performance. Temperature, 2020, vol. 3, pp. 1–10. DOI: 10.1080/23328940.2020.1742056

Tanaka Y., Kanazawa M., Palsson O. S. Increased Postprandial Colonic Motility and Autonomic Nervous System Activity in Patients With Irritable Bowel Syndrome: A Prospective Study. Journal of

Neurogastroenterology and Motility, 2018, vol. 24, no. 1, pp. 87–95. DOI: 10.5056/jnm16216.

Published

2020-12-08

Issue

Section

Pharmaceutical and Food Engineering