THE FORMATION OF FUNCTIONAL AND TECHNOLOGICAL PROPERTIES OF MINCED MEAT UNDER THE INFLUENCE OF ENZYME COMPLEX
Keywords:
enzymes, transglutaminase, bacteria concentrate, minced meat, functional and technological propertiesAbstract
The aim of the work was to study the effect of the transglutaminase enzyme (TG) and bacterial
concentrates on the formation of functional and technological properties of the first-grade
minced beef. The analysis of water binding capacity (WBC) of experimental samples revealed
that the combination of microorganisms and transglutaminase enzymes has a synergistic effect
on the technological properties of minced meat. The maximum values of WBC in samples containing
the TG enzyme were established within 6–12 hours period of aging (59.56–58.49 %), and
in samples containing a set of bacterial cultures and the TG enzyme – within 12–18 hours period
(61.58–57.04 %). The increase in the water-binding capacity of minced meat correlated with a
decrease in weight losses when heated. A significant reduction in weight loss in samples containing
the TG enzyme (individually or in combination with bacterial cultures enzymes) was found.
The lowest weight loss when heated was observed in the samples of minced O-TG in the period
of 6–12 hours (19.03–19.99 %) and in the samples of O-Pro+TG within the12–18 h period
(20.27–21.71 %). In the result of rheological studies, the most pronounced elastic properties in
the samples of O-TG and O-Pro+TG were established, with an increase in elastic deformation
within 24 hours of aging period up to 3.26–3.32 mm values. The maximum strengthening of the
meat structure under the influence of a set of microorganisms and TG enzymes occurred after 12
hours of aging, the level of total deformation reduced to 14.00 mm; the level of plastic deformation
reduced to 10.96 mm. An increase in water binding capacity, reduction of product
weight loss when heated, improvement of both plastic and elastic properties of minced meat
were observed with the use of a set of microorganisms and TG enzymes involved in the binding
of biopolymers of food systems.
References
Ишевский, А.Л. О возможности применения энзимов для получения натуральных полуфабрикатов из мясной обрези / А.Л. Ишевский, В.А. Карлова // Вестник международной академии холода. – 2012. – № 2. – С. 26–28.
Sorapukdee, S. Quality of steak restructured from beef trimmings containing microbial transglutaminase and impacted by freezing and grading by fat level / S. Sorapukdee, P. Tangwatcharin // Asian-Australas Journal of Animal Science. – 2018, vol. 31. – № 1. – P. 129–137.
Extraction and characterisation of pepsinsolubilised collagen from fins, scales, skins, bones and swim bladders of bighead carp (Hypophthalmichthysnobilis) / D. Liu, L. Liang, J.M. Regenstein, P. Zhou // Food Chemistry. – 2012. – № 133. – P. 1441–1448.
Rawdkuen, S. Biochemical and microstructural characteristics of meat samples treated with different plant proteases / S. Rawdkuen,
S. Benjakul // African Journal of Biotechnolo-gy. – 2012. – № 11(76). – P. 14088–14095.
Сафиуллина, Г.И. Трансглютаминаза при производстве фаршированных рулетов / Г.И. Сафиуллина, Л. М. Смертина // Молодежь и наука. – 2017. – № 4.
Kieliszek, M. Microbial transglutaminase and its application in the food industry. A review / M. Kieliszek, A. Misiewicz // Folia Mi-crobiology. – 2014. – № 59. – P. 241–250.
Маджитов, Д.Ф. Трансглютаминаза – эффективно и экономично / Д.Ф. Маджитов // Мясные технологии. – 2011. – № 10. – С. 64.
Uran, H. A research on determination of quality characteristics of chicken burgers produced with transglutaminase supplementation / H. Uran, İ. Yilmaz // Food Science and Technology, Campinas. – 2018. – № 38(1). – P. 19–25.
Atilgan, E. Effects of microbial transglutaminase, fibrimex and alginate on physicochemical properties of cooked ground meat with reduced salt level / E. Atilgan, B. Kilic // Journal of Food Science and Technol-ogy. – 2017. – № 54(2). – P. 303–312.
Tseng, T.F. Purification of transglutaminase and its effects on myosin heavy chain and actin of spent hens / T.F. Tseng, M.T.C. Cheng // Meat Science. – 2002. – № 60(3). – P. 267–270.
Семенова, А.А. Перспективы использования трансглютаминазы для производства мясных продуктов / А.А. Семенова, Е.К. Туниева, С.А. Горбатов // Все о мясе. – 2011. – № 2. – С.14–15.
Курчаева, Е.Е. Использование методов биотехнологии для создания эмульгированных мясных продуктов нового поколения / Е.Е. Курчаева, А.О. Лютикова, Е.С. Мельникова, И.В. Максимов // Актуальные направления научных исследований XXI века: теория и практика. – 2014. – Т. 2, № 4–3. – С. 453–457.
Шукешева, С.Е. Применение стартовых культур при производстве реструктурированных мясных изделий / С.Е. Шукешева, Я.М. Узаков, И.М. Чернуха, Ж.С. Набиева // Вестник Алматинского технологического университета. – 2017. – № 4. – С. 23–26.
Ruiz-Moyano, S. Screening of lactic acid bacteria and bifidobacteria for potential probiotic use in iberian dry fermented sausage / S. Ruiz-Moyano, A. Martin, M.J. Benito // Meat Science. – 2008. – № 80. – P. 715–721.
Hammes, W.P. New developments in meat starter cultures / W.P. Hammes, C. Hertel // Meat Science. – 1998. – № 49. – P. 125–138.
Danylenko, S.G. Selection of microorganisms for fermentation of raw materials / S.G. Da¬nylenko, N.Ph. Kigel, G.V. Burtseva // Biotechnologia Acta. – 2014. – Т. 7, № 4. – P. 107–117. (in Ukr.)
Sanni, A.I. Production of exopolysaccharides by lactic acid bacteria isolated from traditional fermented foods in Nigeria / A.I. Sanni, A.A. Onilude, S.T. Ogunbanwo // Europian Food Research and Technology. – 2002. – № 214. – P. 405–407.
Cerning, J. Production of exopolysaccharides by lactic acid bacteria and dairy propionibacteria / J. Cerning // Lait. – 1995. –
№ 75(4–5). – P. 463–472.
Ruas-Madiedo, P. Screening of exopolysaccharideproducing Lactobacillus and Bifidobacterium strains isolated from the human intestinal microbiota / P. Ruas-Madiedo, J.A. Moreno, N. Salazar // Appl Environ Microbiol. – 2007. – № 73. – P. 4385–4388.
Хамагаева, И.С. Перспективы использования пробиотических микроорганизмов в современной биотехнологии / И.С. Хамагаева // Вестник ВСГУТУ. – 2014. – № 5 (50). – С. 111–116.
El Soda, M. Production of low fat cheddar cheese made using exopolysaccha-ride-producing cultures and selected ripening cultures / M. El Soda // Advances in Microbiology. – 2014. – № 4. – P. 986–995.
Хамагаева, И.С. Создание консорциума пробиотических микроорганизмов с высокой биохимической активностью и экзополисахаридным потенциалом / И.С. Хамагаева, С.Н. Хазагаева, Н.А. Замбалова // Вестник ВСГУТУ. – 2014. – № 1 (46). – С. 97–102.
Assaad, H. Rapid publicationready MS-Word tables for oneway ANOVA / H. Assaad, L. Zhou, R.J. Carroll //Springer Plus. – 2014. – № 3. – P. 474. DOI: 10.1186/2193-1801-3-474.
