INFLUENCE OF ULTRASONIC EXPOSURE WHEN GERMINATING WHEAT GRAIN ON THE SYNTHESIS OF γ-AMINOBUTYRIC ACID
Keywords:
wheat grain germination, synthesis of biologically active substances, γ-aminobutyric acid.Abstract
In the modern world, the use of γ-aminobutyric acid (GABA) as a biologically active component of food products of plant origin is of great interest. Numerous scientific works are devoted to the creation of food products enriched with GABA by seed germination or microbiological fermentation of raw materials. Researchers note that γ-aminobutyric acid is a natural synthesized food ingredient accumulated in plants, capable of exerting an inhibitory effect on the proliferation of cancer cells, an effective regulator of blood pressure; a substance that promotes the functioning of the pancreas and thereby reduces the risk of diabetes. The article considers the possibility of using ultrasonic exposure as a mechanism for intensifying the accumulation of GABA in the process of wheat grain germination. Wheat grains were germinated by controlled germination, as the intensification of the process used grain processing on an acoustic source of elastic vibrations at a frequency of 22 kHz and a power of 340 W (device "Wave" model UZTA-0.4 / 22-OM), exposure time 3 minutes Germination was carried out using drinking water. This method of germination of wheat grain allows you to increase the content of GABA on average 3.2 times. After the digestion procedure is carried out in an in vitro model, the amount of GABA in wheat grain is quite high, which leads to a good value of the bioavailability index of the obtained raw material component. The results of our own research allow us to say that there is a potential for the production of bakery products enriched with GABA by introducing into the recipe raw materials from germinated wheat grains after ultrasound.References
Калинина, И.В. Применение эффектов ультразвукового кавитационного воздействия как фактора интенсификации извлечения функциональных ингредиентов / И.В. Калинина, Р.И. Фаткуллин // Вестник ЮУрГУ. Серия «Пищевые и биотехнологии». – 2016. – Т. 4, № 1. – С. 64–70. DOI: 10.14529/food160108
Науменко, Н.В. Возможности использования биотехнологий при производстве пищевых продуктов / Н.В. Науменко // Актуальная биотехнология. – 2013. – № 2 (5). – С. 14–17.
Потороко, И.Ю. К вопросу обеспечения качества и безопасности воды, используемой в пищевых производствах / И.Ю. Потороко, Р.И. Фаткуллин, И.В. Калинина // Вестник ЮУрГУ. Серия «Экономика и менеджмент». – 2013. – Т. 7, № 1. – С. 165–169.
Потороко, И.Ю. Системный подход в технологии водоподготовки для пищевых производств / И.Ю. Потороко, Р.И. Фаткуллин, Л.А. Цирульниченко // Вестник ЮУрГУ. Серия «Экономика и менеджмент». – 2013. – Т. 7, № 3. – С. 153–158.
Технология и оборудование для обработки пищевых сред с использованием кавитационной дезинтеграции / С.Д. Шеста-ков, О.Н. Красуля, В.И. Богуш, И.Ю. Потороко. – М.: Изд-во «ГИОРД», 2013. – С. 98–102.
Хмелев, В.Н. Многофункциональные ультразвуковые аппараты и их применение в условиях малых производств, сельском и домашнем хозяйстве: монография / В.Н. Хмелев, О.В. Попова. – Барнаул: Изд. АлтГТУ, 1997. – С. 112–126.
Abdou, A.M. Relaxation and immunity enhancement effects of γ-aminobutyric acid (GABA) administration in humans / A.M. Abdou, S. Higashiguchi, K. Horie, M. Kim, H. Hatta, H. Yokogoshi // BioFactors. – 2006. – V. 26. – P. 201–208.
Bai, Q.Y. Effects of components in cul-ture medium on glutamate decarboxylase activity and γ-aminobutyric acid accumulation in foxtail millet (Setaria italica L.) during germination /
Q.Y. Bai, M.Q. Chai, Z.X. Gu, X.H. Cao, Y. Li, K.L. Liu // Food Chem. – 2009. – V. 116. – P. 152–157.
Baranzelli, J. Changes in enzymatic activity, technological quality and gamma-aminobutyric acid (GABA) content of wheat flour as affected by germination / J. Baranzelli, D.H. Kringel, R. Colussi et al. // LWT. – April 2018. – V. 90. – P. 483–490.
Chalermchaiwat, P. Antioxidant activity, free gamma-aminobutyric acid content, selected physical properties and consumer acceptance of germinated brown rice extrudates as affected by extrusion process / P. Chalermchaiwat, K. Jangchud, A. Jangchud et al. // Lebensmittel-Wissenschaft und Technologie Food Science and Technology. – 2015. – V. 64. – P. 490–496.
Cornejo, F. Effects of germination on the nutritive value and bioactive compounds of brown rice breads / F. Cornejo, P.J. Cáce-res, C.M. Villaluenga et al. // Food Chemistry. – 2015. – V. 173. – P. 298–304.
Ding, J. Enhancing contents of γ-aminobutyric acid (GABA) and other micronutrients in dehulled rice during germination under normoxic and hypoxic conditions / J. Ding, T. Yang, H. Feng, M. Dong, M. Slavin, et al. // J. Agric. Food. Chem. – 2016. – Vol. 64 (5). – P. 1094–1102.
Hung, P.V. Phenolic acid composition of sprouted wheats by ultraperformance liquid chromatography (UPLC) and their antiox-idant activities / P.V. Hung, D.W. Hatcher, W. Barker // Food. Chem. – 2011. – Vol. 126 (4). – P. 1896–1901.
Inoue, K. Bloodpressure lowering effect of a novel fermented milk containing gammaaminobutyric acid (GABA) in mild hypertensives 1. / K. Inoue, T. Shirai, H. Ochiai et al. // Eur.
J. Clin. Nutr. – 2003. – V. 57. – P. 490–495.
Junzhou, Ding. Effects of controlled germination on selected physicochemical and functional properties of wholewheat flour and enhanced γ-aminobutyric acid accumulation by ultrasonication / J. Ding, G.G. Hou, B.V. Nemzer et al. // Food Chemistry. – 2018. – V. 243. – P. 214–221.
Ohm, J. Ascorbic acid-A potential oxidant scavenger and its role in plant development and abiotic stress tolerance / J. Ohm, C.W. Lee, K. Cho // Cereal Chemistry. – 2016. – V. 93. – P. 612–617.
Palmer, L.M. The cellular basis of GABAB-mediated interhemispheric inhibition / L.M. Palmer, J.M. Schulz, S.C. Murphy et al. // Science. – 2012. – V. 335. – P. 989–993.
Park, J.K. Influence of ginsenosided on the kainic acid-induced seizure activity in immature rats / J.K. Park, S.H. Jin, J.H. Ko et al. // Journal of Biochemistry and Molecular Biology. – 1999. – V. 32. – P. 339–344.
Rodríguez-Roque, M.J. Impact of food matrix and processing on the in vitro bioaccessibility of vitamin C, phenolic compounds, and hydrophilic antioxidant activity from fruit juice-based beverages / M.J. Rodríguez-Roque, B. de Ancos, C. Sánchez-Moreno et al. // Journal of Functional Foods. – 2015. – Vol. 14. – P. 33−43.
Rossetti, V. Determination of glutamate decarboxylase by high performance liquid chromatography / V. Rossetti, A. Lombard // Journal of Chromatography B. – 1996. – V. 681. – P. 63–67.
Scott-Taggart, C.P. Regulation of gamma-aminobutyric acid synthesis in situ by glutamate availability / C.P. Scott-Taggart, O.R. Van Cauwenberghe, M.D. McLean, B.J. Shelp // Physiol. Plant. – 1999. – V. 106. – P. 363–369.
Tian, B. Physicochemical changes of oat seeds during germination / B. Tian, B. Xie, J. Shi, J. Wu, Y. Cai, et al.// Food. Chem. – 2010. – Vol. 119 (3). – P. 1195–1200.
Wu, Z. Food grade fungal stress on germinating peanut seeds induced phytoalexins and enhanced polyphenolic antioxidants / Z. Wu, L. Song, D. Huang // J. Agric Food. Chem. – 2011. – V. 59. – P. 5993–6003.
Yang, H. The ultrasoundtreated soybean seeds improve edibility and nutritional quality of soybean sprouts / H. Yang, J. Gao, A. Yang, H. Chen // Food Res. Int. – 2015. – V. 77. – P. 704–710.





