METHODOLOGY FOR OBTAINING COMPLEX BIOACTIVE SUBSTENCES FROM PLANT RESOURCES

Аннотация

Natural biologically active substances obtained from various plant sources are highly valued for their low toxicity, wide range of sources and high yield of final components. It has been established that biologically active (antioxidant) properties are characterized by such biochemical substances as flavonoids, polyphenols, vitamins, especially carotenoids, and active polysaccharides. Rosemary contains significant concentrations of biologically active components, the most important of which are essential oils, carnosol, carnosic acid and rosemary acid. As a rule, there is a synergistic effect between different types of antioxidants. The antioxidant capacity of bioactive components after mixing can be significantly higher than that of the original antioxidants, and it is more effec-tive, less energy-consuming, safer and less toxic. Rosemary leaf extracts can enhance their antioxidant activity when interacting with natural carotenoids. The purpose of this study was to design and analyze the optimized parameters for obtaining a complex of biologically active substances based on the principles of synergism of carrot vitamins and rosemary polyphenolic compounds. To confirm and comparatively analyze the bioactive properties of the obtained complex extracts, studies of antiradical activity, content of polyphenolic and flavonoid compounds were performed. The monoextracts based on 70 % ethanol as well as distilled water extract of rosemary were characterized by the highest DPPH activity and significant concentration of polyphenolic substances. The ef-ficiency of complex plant ethanol extracts was established, the highest concentration of bioactive components and antioxidant activity in the range of 86.9–92.5 % was observed. As a result of this study, the feasibility of application of different extraction agents for the isolation of bioactive compounds from rosemary and carrots was scientifically substantiated, and bioactive components with proven antioxidant properties were developed.